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scientist Arne Tiselius developed an apparatus which could be used to measure values of proteins (see Figure 5.2). In this system, proteins moved towards the electrode of opposite charge as an ascending and descending boundary in a U-shaped tank. While this could separate proteins into moving boundaries allowing mobility measurements to be made, the resolution of the system was extremely low, large volumes of protein were required and the apparatus used was quite cumbersome. Zone electrophoresis was then introduced which involved separation of biomolecules in some form of solid support. Examples of these supports include paper, cellulose and gel networks. These supports have considerable mechanical strength and allow staining of biomolecules after separation. Most modern electrophoretic separations take place in zonal gel electrophoresis systems. More recently, it has been found that electrophoresis in very thin capillaries allows the use of higher than normal voltages with ef cient heat dissipation giving very high resolution. Capillary electrophoresis is a versatile analytical technique capable of separating a wide range of biological samples such as biomolecules, viruses and even whole cells (Section 5.10). A wide range of zonal and free solution systems are possible in capillary electrophoresis making an interesting historical link with the pioneering work of Tiselius.

Data storage overview Loading and saving objects Example: moving objects across a network Using database-like storage Converting to and from C structures Converting data to standard formats Compressing data

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Source: (From Kernec et al., 1996) Kernec, F et al., (1996) Biochemical and Biophysical Research Communication s 225, 819 825 Reproduced with permission of Academic Press, Inc

Quick Start: Drawing a Window . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1: Starting to Draw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

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INSERT INTO table VALUES ( value1, value2, ... );

Uninterruptible Power Supply (UPS)

Coherence From quantum physics, coherence is a property exhibited by matter and energy when it remains unmeasured and is thus able to exist in more than one state simultaneously (superposition) The wave function is a math formula that describes all possible futures and possible pasts for a system Until a measurement is made, the system is coherent and all possibilites are both true and false Once a measurement is made, the wave function collapses and the state becomes determined Any interaction can be considered a measurement, which is why coherence is such a delicate property to maintain [661] Collider Accelerator in which two beams traveling in opposite directions are steered together to provide high-energy collisions between the particles in one beam and those in the other [50] Colliding-beam experiments Experiments done at colliders.

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